B1090 

KRACKE BLOOD CULTURE MEDIUM

Formula

Ingredients 

gms/lit.

Beef heart solids

Beef brain solids 

Proteose peptone 

Sodium chloride 

Dextrose 

Sodium citrate 

Disodium phosphate 

2.00

1.00

10.00

49.00

10.00

1.00

2.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 3.75 gms. in 50ml. distilled water. Allow the suspension to stand for 15 minutes. When all the meat particles are thoroughly wetted, sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes.

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