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B1089I |
KLIGLER IRON AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzyme hydrolysate Beef extract Yeast extract Proteose peptone Lactose Glucose anhydrous Ferrous ammonium sulphate,6H2O Sodium chloride Sodium thiosulphate,5 H2O Phenol red Agar |
20.00 3.00 3.00 5.00 10.00 1.00 0.50 5.00 0.50 0.025 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 57.7 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Mix well & distribute into tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes to set as slopes with 1 inch butts.Best reactions are obtained on freshly prepared media. |
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