B1087 

KIRSCHNER MEDIUM BASE, MODIFIED

Formula

Ingredients 

gms/lit.

Disodium phosphate

Monopotassium phosphate 

Magnesium sulphate 

Sodium citrate

L-aspargine 

3.00

4.00

0.60

2.50

5.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 15.1 gms. in 700ml. distilled water. Add 200 ml glycerol. Heat to boiling to dissolve the medium completely. Dispense in 9 ml aliquots. Sterilize by autoclaving at 10 lbs pressure (115C) for 15 minutes. Just before use, aseptically add 1 ml of Horse serum (BF021) and 100 IU Penicillin per 9ml medium.

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