B1086 

KINGS OF MEDIUM BASE

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Phenol red 

Agar 

0.20

0.003

0.30

Final pH (at 25C) : 7.4 + 0.2

Directions

Suspend 0.5 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool to 45-50C and aseptically add filter sterilized solution of desired carbohydrate to get a final concentration of 1% and dispense in sterile tubes.

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