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B1085 |
LEE’S AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Lactose Sucrose Calcium carbonate Dipotassium phosphate Bromo cresol purple Agar |
10.00 10.00 5.00 5.00 3.00 0.50 0.02 18.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 51.52 gms. in 1000ml. distilled water. Heat gently to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. While dispensing, mix carefully to suspend calcium carbonate evenly. Pour into sterile petri plates to obtain 4 -5 mm thick gel. |
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