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B1079 |
KAPER’S MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Casein enzymic hydrolysate Yeast extract L-Ornithine hydrochloride Mannitol Inositol Sodium thiosulphate Ferric ammonium citrate Bromo cresol purple Agar |
5.00 10.00 3.00 5.00 1.00 10.00 0.40 0.50 0.02 3.00 |
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Final pH (at 25°C) : 6.7 + 0.2 |
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Directions |
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Suspend 38 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes (5ml) and sterilize by autoclaving at 15 lbs pressure (121°C) for 12 minutes. |
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