B1068 

ITC BROTH BASE

Formula

Ingredients

gms/lit

Casein enzymic hydrolysate

Yeast extract

Magnesium chloride, 6H2O

Sodium chloride

Malachite green 

Irgasan (Trichlosan)

10.00

 1.00

60.00

5.00

0.01

0.001

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 76 gms in 988 ml distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45- 50°C.Aseptically add 1 vial of each Ticarcillin Supplement (BF065) and Potassium Chlorate Supplement (BF066). Mix well before dispensing in sterile tubes.

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