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B106 |
AZIDE BLOOD AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptone, special Beef extract Sodium chloride Sodium azide Agar |
10.00 3.00 5.00 0.20 15.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 33.2 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C. For preparing Blood Agar Base 5% w/v sterile defibrinated blood is added aseptically. |
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