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B1058 |
HEMMES MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Dextrose Lactose Sucrose Urea Sodium chloride Ferrous sulphate Sodium thiosulphate Phenol red Agar |
10.00 3.00 0.30 10.00 10.00 2.00 4.00 0.04 0.10 0.015 5.50 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 45 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 5 ml. amounts in 12 mm tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in slanted position to give a butt of 3 cm and slant of 2 cm or in an upright position. |
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