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B1050 |
GTC AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Sodium chloride Monopotassium phosphate Dextrose Esculin Thallous acetate Ferric citrate Polysorbate 80 Agar |
15.00 5.00 5.00 5.00 1.00 1.00 0.50 0.50 0.75 15.00 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 24.37 gms. in 500 ml. distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C and aseptically add rehydrated contents of 1 vial of GTC Supplement and 10ml of sterile 10% sodium bicarbonate solution just before use. Mix well and pour into sterile petri plates. |
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