B104 |
ANAEROBIC AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Dextrose Sodium chloride Sodium thioglycollate Sodium formaldehyde sulphoxylate Methylene blue Agar |
20.00 10.00 5.00 2.00 1.00 0.002 20.00 |
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Final pH (at 25°C): 7.2 + 0.2 |
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Directions |
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Suspend 58 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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