B104

ANAEROBIC AGAR

Formula

Ingredients

gms/lit.

Casein enzymic hydrolysate 

Dextrose

Sodium chloride

Sodium thioglycollate

Sodium formaldehyde sulphoxylate

Methylene blue

Agar  

20.00

10.00

5.00

2.00

1.00

0.002

20.00

Final pH (at 25°C):  7.2 + 0.2

Directions 

Suspend 58 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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