B1039 |
FLETCHER LEPTOSPIRA MEDIUM BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Sodium chloride Agar |
0.30 0.20 0.50 1.50 |
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Final pH (at 25°C) : 7.9 + 0.2 |
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Directions |
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Suspend 2.5 gms. in 920 ml. distilled water (volume of water should be appropriate to compensate addition of enrichment).Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add 80 ml sterile Leptospira Enrichment (Rabbit/Horse serum) to make final volume to 1 litre. |
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