B1039 

FLETCHER LEPTOSPIRA MEDIUM BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Beef extract 

Sodium chloride 

Agar 

0.30

0.20

0.50

1.50

Final pH (at 25°C) : 7.9 + 0.2

Directions 

Suspend 2.5 gms. in 920 ml. distilled water (volume of water should be appropriate to compensate addition of enrichment).Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add 80 ml sterile Leptospira Enrichment (Rabbit/Horse serum) to make final volume to 1 litre.

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