B1029 

ESCULIN AZIDE BROTH

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Yeast extract 

Bile salts 

Sodium chloride 

Esculin 

Ferric ammonium citrate 

Sodium azide 

20.00

5.00

10.00

1.00

1.00

0.50

0.25

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 37.8 gms. in 1000 ml. distilled water. Heat, if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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