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B1027 |
ENTAMOEBA MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Liver infusion from Proteose peptone Sodium β -glycerophosphate Sodium chloride Agar |
272.00 5.50 3.00 2.70 11.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 33 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Distribute in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow tubes to solidify in a slanted position.Cover about half of the slant with fresh sterile horse serum-saline mixture (1:6) and add a 5 mm loopful of rice powder which has been sterilized in an oven at 160°C for one hour.SCORCHING OF THE MEDIUM SHOULD BE PREVENTED. |
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