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B1026 |
ENRICHED THIOGLYCOLLATE BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Dextrose Sodium chloride Sodium thioglycollate L-Cystine Sodium sulphite Hemin Vitamin K1 Agar |
17.00 3.00 6.00 2.50 0.50 0.25 0.10 0.005 0.001 0.70 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 30 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 12 –15 lbs pressure (118 – 121°C) for 15 minutes. Cool and dry under 85% N2, 10% H2 and 5% CO2 atmosphere. |
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