B1023 

ENDO AGAR WITH NaCl

Formula

Ingredients 

gms/lit.

Special peptone

Lactose 

Sodium chloride 

Dipotassium phosphate 

Sodium sulphite 

Basic fuchsin (pararosaniline) 

Agar 

8.00

10.00

3.00

2.00

2.50

0.20

12.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 37.7 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If the solidified culture medium is somewhat too red, then to remove the colour, add few drops (Max. 1 ml/lit.) of a freshly prepared 10% sulphite solution and boil.

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