B1023 |
ENDO AGAR WITH NaCl |
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Formula |
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Ingredients |
gms/lit. |
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Special peptone Lactose Sodium chloride Dipotassium phosphate Sodium sulphite Basic fuchsin (pararosaniline) Agar |
8.00 10.00 3.00 2.00 2.50 0.20 12.00 |
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Final pH (at 25°C) : 7.5 + 0.2 |
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Directions |
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Suspend 37.7 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If the solidified culture medium is somewhat too red, then to remove the colour, add few drops (Max. 1 ml/lit.) of a freshly prepared 10% sulphite solution and boil. |
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