B101 

A C AGAR 

Formula 

B101

Ingredient

grm/lit

Proteose peptone

Beef extract

Yeast extract 

Malt extract

Dextrose 

Ascorbic acid 

Agar 

20.00

3.00

3.00

3.00

5.00

0.20

1.00

 

Final pH (at 25°C) : 7.2 + 0.2 

Directions :

Suspend 35.2 gms. of B101 in 1000 ml. distilled water.  Heat to boiling to dissolve the medium completely. Distribute in tubes or bottles to give the desired depth and sterilize by autoclaving at 15 lbs pressure (121° C) for 20 minutes.

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