B098 |
LISTERIA IDENTIFICATION BROTH BASE (PALCAM) |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Yeast extract Lithium chloride Esculin Ammonium ferric citrate D-Mannitol Soya lecithin Polysorbate 80 Phenol red |
23.00 5.00 10.00 0.80 0.50 5.00 1.00 2.00 0.08 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 23.7 gms. in 500ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile reconstituted contents of 1 vial of Listeria Selective Supplement (BF072). Mix well before pouring. |
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