B098 

LISTERIA IDENTIFICATION BROTH BASE (PALCAM)

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Yeast extract 

Lithium chloride 

Esculin 

Ammonium ferric citrate 

D-Mannitol 

Soya lecithin 

Polysorbate 80 

Phenol red 

23.00

5.00

10.00

0.80

0.50

5.00

1.00

2.00

0.08

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 23.7 gms. in 500ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile reconstituted contents of 1 vial of Listeria Selective Supplement (BF072). Mix well before pouring.

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