B081

THIOL MEDIUM

Formula 

B081

Ingredients 

gms/lit.

Proteose peptone 

Yeast extract 

Dextrose 

Sodium chloride 

Thiol compound 

p-amino benzoic acid 

Agar 

10.00

5.00

1.00

5.00

8.00

1.00

1.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 30 gms. of B081 in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes or flasks to a depth of 6 cm for neutralization of Penicillin or in shallow layers for neutralization of streptomycin. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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