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B056 |
VAGINALIS AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptic digest of animal tissue Beef extract Yeast extract Corn starch Sodium chloride Agar |
12.00 15.00 3.00 3.00 1.00 5.00 13.50 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 52.5 gms. in 950ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 95ml amounts and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to around 50-55°C and aseptically add 5ml of sterile anticoagulated human blood to every 95ml sterile basal medium. Mix well and pour into sterile petri plates. |
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