B045 |
THIOGLYCOLLATE MEDIUM W/O DEXTROSE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Sodium chloride Dipotassium phosphate Sodium thioglycollate L-cystine Sodium sulphite Methylene blue Agar |
20.00 2.50 1.50 0.60 0.40 0.20 0.002 0.50 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 25.7 gms. in 1000ml. distilled water. If the medium is to be used for fermentation studies for diagnostic work add 0.5 to 1% carbohydrate of choice. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively, sterile carbohydrate solutions may be added to the broth after sterilization. The prepared medium should be stored in the dark at room temperature. |
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