B045 

THIOGLYCOLLATE MEDIUM W/O DEXTROSE

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium chloride

Dipotassium phosphate

Sodium thioglycollate

L-cystine

Sodium sulphite

Methylene blue

Agar

20.00

2.50

1.50

0.60

0.40

0.20

0.002

0.50

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 25.7 gms. in 1000ml. distilled water. If the medium is to be used for fermentation studies for diagnostic work add 0.5 to 1% carbohydrate of choice. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively, sterile carbohydrate solutions may be added to the broth after sterilization. The prepared medium should be stored in the dark at room temperature.

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