B043 

THIOGLYCOLLATE MEDIUM W/ HEMIN & VITAMIN K

Formula

Ingredients 

gms/lit.

Tryptose

Yeast extract

Sodium thioglycollate

Sodium chloride

L-cysteine hydrochloride

Sodium bicarbonate

Resazurin

Hemin

Vitamin K

Agar

15.00

10.00

0.50

2.50

0.50

0.40

0.001

0.005

0.0005

0.75

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 29.7 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Print This Page

Close Window