B043 |
THIOGLYCOLLATE MEDIUM W/ HEMIN & VITAMIN K |
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Formula |
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Ingredients |
gms/lit. |
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Tryptose Yeast extract Sodium thioglycollate Sodium chloride L-cysteine hydrochloride Sodium bicarbonate Resazurin Hemin Vitamin K Agar |
15.00 10.00 0.50 2.50 0.50 0.40 0.001 0.005 0.0005 0.75 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 29.7 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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