B041

VIOLET RED BILE BROTH

Formula 

B401

Ingredients 

gms/lit.  

Peptic digest of animal tissue

Yeast extract 

Lactose 

Bile salt mixture 

Sodium chloride 

Neutral red

Crystal violet 

 

7.00

3.00

10.00

1.50

5.00

 0.03

0.002

 

 

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 26.53 gms. of B041 in 1000 ml. distilled water. Heat with stirring to boiling to  dissolve the medium completely. DO NOT AUTOCLAVE Cool to 45°C and pour into sterile petri plates containing the inoculum.

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