B039 

SOYABEAN CASEIN DIGEST AGAR 

(TRYPTONE SOYA AGAR)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Papaic digest of soyabean meal

Sodium chloride 

Agar 

15.00

5.00

5.00

15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 40 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, aseptically add 5% v/v defibrinated blood in previously cooled to 45-50°C

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