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B032 |
POTASSIUM CYANIDE BROTH BASE without KCN |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Disodium phosphate Monopotasium phosphate Sodium chloride |
3.00 5.64 0.225 5.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 13.9 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Dispense in 100 ml. amounts and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add sterile 1.5 ml. of 0.5% potassium cyanide solution to each 100 ml. of basal medium. Mix thoroughly and dispense in 1 ml. amounts. |
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