B028

WILSON BLAIR AGAR BASE WITH BG

Formula

Ingredients

gms/lit.

Peptic digest of animal tissue

Beef extract 

Dextrose 

Disodium phosphate 

Ferrous sulphate 

Bismuth sulphite indicator 

Brilliant green 

Agar 

10.00

5.00

5.00

4.00

0.30

8.00

0.025

20.00

Final pH (at 25°C) : 7.7 + 0.2

Directions 

Suspend 52 gms. in 1000ml. distilled water. Heat gently with frequent agitation until the medium is dissolved completely. DO NOT AUTOCALVE. Cool to 50-55°C. Mix well to disperse precipitate and pour thick plates (25 ml medium per plate).Dry the plates before use, avoiding over drying.

Print This Page

Close Window