B024 

FLUID LACTOSE MEDIUM

Formula

Ingredients 

gms/lit.

 Pancreatic digest of gelatin 

Beef extract 

Lactose 

5.00

3.00

5.00

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 13 gms. in 1000 ml distilled water. Mix well and distribute into tubes with inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. The concentration of medium is adjusted in accordance with sample size.

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