B022

EMB AGAR

Formula 

B022 

Ingredients 

gms/lit.

Peptic digest of animal tissue

Dipotasium phosphate 

Lactose 

Sucrose 

Eosin-Y 

Methylene Blue

Agar 

10.00 

2.00 

5.00 

5.00 

0.40 

0.065 

13.50 

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 36.00 gms. B022 in 1000 ml. distilled water. Heat to dissolve the medium completely Dispense & sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVER HEATING. Cool to 50°C & shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) & to suspend the flocculent precipitate. (If EMB agar is inoculated on the same day, it may be used without autoclave sterilization)

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