B017 

CARY – BLAIR MEDIUM

Formula

Ingredients 

gms/lit.

Disodium phosphate

Sodium thioglycollate 

Sodium chloride 

Agar 

1.10

1.50

5.00

5.00

Final pH (at 25°C) : 8.4 + 0.2

Directions 

Suspend 12.6 gms. in 991 ml. distilled water. Boil to dissolve the medium completely. Cool to 50°C and aseptically add 9 ml of 1% aqueous calcium chloride solution. Adjust pH to 8.4 if necessary. Distribute in 7 ml. amounts in screw capped tubes. Steam for 15 minutes. Cool and tighten the caps.

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