B012 

BISMUTH SULPHITE AGAR (AS PER I.P.)

Formula

Ingredients 

gms/lit.

Peptone

Beef Extract 

Dextrose,monohydrate 

Disodium hydrogen phosphate Anhydrous

Sodium sulphite 

Ferrous citrate

Bismuth ammonium citrate 

Brilliant Green 

Agar 

10.00

6.00

5.00

4.00

10.00

 0.40

3.00

0.01

24.00

Final pH (at 25°C) : 7.7 + 0.2

Directions 

Suspend 63.40 grams in 1000ml distilled water. Boil to dissolve the medium completely. Mix well to disperse suspension and pour plates.DO NOT AUTOCALVE OR OVERHEAT.

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