B003I

YERSINIA IDENTIFICATION BROTH BASE

Formula

Ingredients 

gms/lit.

L-tryptophan

Monopotassium phosphate

Dipotassium phosphate

Sodium chloride

Phenol red

3.00

1.00

1.00

5.00

0.025

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 10 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add Urea solution (BF048). Mix well before dispensing in sterile tubes.

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