B001I

YERSINIA SELECTIVE AGAR BASE

Formula

Ingredients 

gms/lit.

Peptone,special

Yeast extract 

Mannitol 

Sodium pyruvate 

Sodium chloride 

Magnesium sulphate 

Sodium deoxycholate 

Neutral red 

Crystal violet 

Agar 

20.00

2.00

20.00

2.00

1.00

0.01

0.50

0.03

0.001

12.50

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 29 gms. in 500ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add reconstituted contents of 1 vial of Yersinia Selective supplement. Mix well before pouring into sterile petri plates.

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